Beta-galactosidase activity assay for yeast
نویسندگان
چکیده
منابع مشابه
Quantitative beta-galactosidase assay suitable for high-throughput applications in the yeast two-hybrid system.
Measurement of beta-galactosidase (beta-gal) activity is an important step in every yeast two-hybrid assay, yet many commonly used methods have distinct disadvantages, such as being only qualitative, time-consuming, and cumbersome when processing large numbers of samples. To overcome these drawbacks, we have implemented a novel technique, termed pellet X-gal assay, that allows simultaneous quan...
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mouse genotyping by the three-primer multiple PCR. However, both of the smaller PCR products of 1068 (specific for the wild-type allele of mGluR4) and 1170 bp (specific for the mGluR4 transgene) were visible. Thus, all three allele combinations were clearly distinguishable on agarose gels stained with ethidium bromide. DNA from wildtype mice give a single smaller band (1068 bp; see Figure 2B, l...
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(shaded bars; left scale) are presented beside β-gal activity units determined on the indicated strains in the same conditions by ONPG-based assays (empty bars; right scale), performed using the traditional Miller method for bacteria (3) and crude protein extracts for yeast (1). MUG, 4-methylumbelliferyl β-d-galactopyranoside; ONPG, o-nitrophenyl-β-d-galactopyranoside; β-gal; β-galactosidase. B...
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Two enzymes having lactase activity are present in the equine small intestine. The first, the digestive enzyme, neutral beta-galactosidase, declines in activity from birth to three years, disappearing completely between 3 and 4 years of age. The other, the soluble lysosomal enzyme, acid beta-galactosidase, having affinity for lactose and a synthetic beta-galactoside, shows a decrease in activit...
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To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between ...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2019
ISSN: 2043-0116
DOI: 10.1038/protex.2019.002